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ECO-FRIENDLY APPROACH TO LIVER PROTECTION: AVOCADO SEED EXTRACT AGAINST CCL₄ TOXICITY

Authors: Abd El-Rahman Ibrahim and Sherif Rabea Mohamed

Received March 14, 2025

Accepted for publication April 30, 2025

Published June 30, 2025. Volume 3:1 Pages 180—191

Checked for plagiarism Yes

Peer reviewer comments 2

Correspondence: ia.mona2012@yahoo.com

Abstract

The current study examined the potentially protective effects of aqueous avocado seed extract (ASE) against mice's liver damage brought on by carbon tetrachloride (CCl₄). Four groups of twenty male mice were created: ASE + CCl₄, ASE, CCl₄, and control. Serum levels of alanine aminotransferase (ALT) with aspartate aminotransferase (AST) were measured, and histological, Mallory's trichrome, and caspase-3 immunohistochemical analyses were performed to evaluate hepatic function. The treatment of CCl₄ resulted in collagen buildup, significant histological changes, enhanced caspase-3 expression, and noticeably elevated ALT in addition to AST values. By considerably reducing these biochemical and structural alterations, ASE treatment helped to return the liver architecture to normal. ASE by itself had no negative impacts. These results imply that ASE has hepatoprotective properties, most likely via anti-apoptotic and antioxidant pathways, and could be utilized as a natural remedy to prevent liver damage brought on by chemicals.